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Peculiarities of extraction of β-lactoglobuline in protein mineral concentrates at electroactivation of whey

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dc.contributor.author BOLOGA, Mircea
dc.contributor.author VRABIE, Elvira
dc.contributor.author PALADII, Irina
dc.contributor.author ILIASENCO, Olga
dc.contributor.author STEPURINA, Tatiana
dc.contributor.author VRABIE, Valeria
dc.contributor.author POLICARPOV, Albert
dc.contributor.author SPRINCEAN, Catalina
dc.date.accessioned 2022-01-05T10:34:41Z
dc.date.available 2022-01-05T10:34:41Z
dc.date.issued 2021
dc.identifier.citation BOLOGA, Mircea, VRABIE, Elvira, PALADII, Irina et al. Peculiarities of extraction of β-lactoglobuline in protein mineral concentrates at electroactivation of whey. In: One Health & Risk Management. 2021, V. 2, Iss. 1, pp. 52-68. ISSN 2587-3458, 2587-3466. en_US
dc.identifier.issn 2587-3458
dc.identifier.issn 2587-3466
dc.identifier.uri https://doi.org/10.38045/ohrm.2021.1.06
dc.identifier.uri http://repository.utm.md/handle/5014/18686
dc.description.abstract Introduction. Whey is a by-product and an excellent source of proteins that is rather aggressive due to a large amount of organic substances it contains. The electro-activation of whey applied in the experiments is a wasteless method that allows the valorification of all whey components. β-lactoglobulin (β-Lg) makes up 50% of the whey proteins and 12% of the total protein content in milk. Material and methods. The recovery of β-Lg in protein-mineral concentrates (PMC) by electro-activation processing of different types of whey with different initial protein content was investigated in seven configurations. The recovery of protein fractions in the PMCs were analyzed via electrophoresis with sodium dodecyl sulfate (SDS-PAGE) and 15% non-denaturing polyacrylamide gel (PAAG). Results. Whey electro-fractionation and the obtaining of PMCs with predetermined protein content, namely of β-Lg, were studied on three whey types, processed at different treatment regimens and in seven configurations. The proper management of electroactivation by varying the treatment regimens will allow the electro-fractionation of different types of dairy by-products. Conclusions. The maximum amount of β-Lg recovered in PMCs on electroactivation is 66-71% depending on the processed whey and on the treatment regimens. Obviously, the extraction of β-Lg from initially lower protein content shows a higher recovery degree of β-Lg. The registered temperatures allows formation of PMCs without thermal denaturation. en_US
dc.description.abstract Introducere. Zerul, ca unul dintre subprodusele lactate, fiind o sursa excelenta de proteine, se prezinta, de asemenea, ca un produs agresiv, din cauza substanțelor organice deținute în cantitați mari. Electroactivarea zerului este o metoda non-reziduala care permite valorificarea tuturor componentelor din zer. ß-lactoglobulina (ß-Lg) reprezinta 50% din proteinele din zer .i 12% din con.inutul total de proteine ale laptelui. Material și metode. Extragerea ß-Lg în concentrate proteice minerale (CPM), la electroactivarea diverselor tipuri de zer, cu un conținut proteic inițial diferit, a fost cercetata în 7 configurații. Fracțiile proteice extrase din zer în CPM au fost analizate prin electroforeza cu dodecil sulfat de sodiu (SDS-PAGE) și concentrația gelului de poliacrilamida ne denaturant (PAAG) de 15%. Rezultate. Electrofracționarea zerului și obținerea CPM cu un conținut proteic predeterminat, și anume cu ß-Lg, a fost demonstrata la prelucrarea a trei tiputi de zer, în regimuri de tratament diferite, cercetate în 7 configurații. Gestionarea corecta a electroactivarii, cu variația regimurilor de tratament, permite electrofracționarea diferitelornsubproduse lactate. Concluzii. Cantitatea maxima de ß-Lg, extrasa în CPM la electroactivare, constituie 66-71%, în funcție de zerul procesat și de regimurile de tratare. Este evident ca volumul de extragere a ß-Lg din zerul cu un conținut proteic inițial mai mic, în CPM este mai mare. Temperaturile înregistrate permit formarea CPM fara denaturare termica. en_US
dc.language.iso en en_US
dc.publisher Moldovan Association for Biosafety and Biosecurity en_US
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/us/ *
dc.subject whey en_US
dc.subject protein-mineral concentrates en_US
dc.subject lactoglobulin en_US
dc.subject zer en_US
dc.subject concentrate proteice en_US
dc.subject proteine en_US
dc.subject lactoglobulină en_US
dc.title Peculiarities of extraction of β-lactoglobuline in protein mineral concentrates at electroactivation of whey en_US
dc.title.alternative Particularita.ile extragerii ß-lactoglobulinei în concentrate proteice minerale la electroactivarea zerului en_US
dc.type Article en_US


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Attribution-NonCommercial-NoDerivs 3.0 United States Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States

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