The influence of some metal nanooxides on the exocellular amylase activity of Aspergillus niger CNMN Fd 06 mycelial fungal strain

Abstract

In order to identify new innovative solutions for directing and increasing amylase biosynthesis in fungal strain Aspergillus niger CNMN FD 06, the effect of nanocomposites of Ti, Fe, Zn and Cu metals with different characteristics was evaluated. In the research were included the nanoparticles (NPs) as follow: nanodioxide of Ti (TiO2) with dimensions of 21 nm and <100 nm, titanium silicon oxide nanoparticles TiSiO4<50 nm, nanooxides Fe3O4 50-100 nm, ZnO ≤50 nm and CuO <50 nm, as well as Cu metal (99,5%) with sizes of < 60-80 nm. The amylolytic activity of the strain Aspergillus niger CNMN FD 06 was monitored during the 5-7th days of cultivation – a period that corresponds to the maximum manifestation of amylase activity in the producer under submerged cultivation in classic conditions (control, without NPs). The nanocomposites were included in the culture medium in concentrations of 5 mg/L, 10 mg/L and 15 mg/L.